| In order to investigate the functions of certain biomaterials in human stem cell behavior, biosurface engineering techniques such as microcontact printing and microfluidics can be applied to replicate the microenvironment in which these cells reside. During microcontact printing processes, the transfer of these biomolecules is influenced by the changes in surface energy and surface tension. To analyze these changes, contact angle measurements were performed at each stage of microcontact printing by using a rame hart goniometer. These measurements were taken on glass slides and polydimethylsiloxane, at various temperatures and after certain plasma treatments and cleaning techniques. The surface tensions were then calculated using Young’s relation. . This information will enable us to determine the optimal conditions for the transfer of these biomolecules, which can be incorporated into the microcontact printing technique for producing high-resolution patterns and maximizing the efficiency of this process. |